AKT1 Antibody

Code: CSB-PA655043XA01DOA

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0.1ml

0.1ml1ml

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Species Reactivity: Arabidopsis thaliana

Raised in: Rabbit

Application: ELISA, WB (ensure identification of antigen)

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Target Background

Product Details

Uniprot NO.

Q38998

Target Names

AKT1

Alternative Names

AKT1 antibody; At2g26650 antibody; F18A8.2Potassium channel AKT1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana AKT1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Tested Applications

ELISA, WB (ensure identification of antigen)

Lead Time

Made-to-order (14-16 weeks)
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

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Target Background

Function(From Uniprot)

Highly selective inward-rectifying potassium channel that mediate potassium uptake by plant roots in response to low K(+) conditions, by a calcium-, CBL-, and CIPK-dependent pathway. Positively regulated by phosphorylation by CIPK23. Negatively regulated by a kinase-independent regulatory mechanism involving a competing direct binding of CBL10. Involved in the stomatal regulation by monitoring the turgor pressure in guard cells. Assuming opened or closed conformations in response to the voltage difference across the membrane, the channel is activated by hyperpolarization. May interact with the cytoskeleton or with regulatory proteins. Is essential with POT5/HAK5 for high-affinity potassium uptake in roots during seedling establishment and postgermination growth under low potassium conditions.

Gene References into Functions

CIPK23 and AtKC1 exhibit distinct effects; however, they act synergistically and balance K(+) uptake/leakage to modulate AKT1-mediated low potassium responses in Arabidopsis. PMID:26829980
results suggest that NO decreases K(+) absorption by promoting the synthesis of vitamin B6 PLP, which further represses the activity of K(+) channel AKT1 in Arabidopsis. PMID:25355908
Examination of the athak5 atakt1 double mutant, revealing novel aspects of an uptake system as yet unidentified by genetic means. PMID:23553635
AKT1 is regulated by CIPK23 in guard cells and is involved in water stress responses. PMID:22210899
These findings provide further insights into the signaling network consisting of CBL-CIPK-PP2C interactions in the activation of the AKT1 channel. PMID:21596690
Electrophysiological results showed that AtKC1 inhibited the AKT1-mediated inward K(+) currents and negatively shifted the voltage dependence of AKT1 channels. PMID:20514083
AtHAK5 and AKT1 are vital for plant growth and development at low K+ concentrations. PMID:20413648
In the range between 0.01 and 0.05 mM K+ AtHAK5 and AtAKT1 are the only contributors to K+ acquisition. At higher K+ concentrations, unknown systems come into operation and participate together with AtAKT1 in low-affinity K+ uptake. PMID:20088908
Potassium transport and gene expression in A. thaliana deficient at this site. PMID:15734909
CIPK23 directly phosphorylates the K+ transporter AKT1 PMID:16814720
Data show that interacting calcium sensors (CBL1 and CBL9) together with CIPK23, but not either alone, activated the AKT1 channel in a Ca(2+)-dependent manner, connecting the Ca(2+) signal to K(+) uptake through activation of a K(+) channel. PMID:16895985
AtAKT1 mediates K+ absorption at high concentrations, and remains active in the presence of NH4+ although activity is reduced. PMID:19000196
AtKC1-AKT1 channels in Arabidopsis roots facilitate growth under K+-limiting conditions PMID:19509299

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

Potassium channel family, Plant (TC 1.A.1.4) subfamily

Tissue Specificity

Preferentially expressed in the peripheral cell layers of root mature including root cortex and root hairs. Detected also, at a lower level, in the mesophyll of the leaves and at restricted sites corresponding to hydathodes and guard cells.

Database Links

UNIGENE: At.48521

KEGG: ath:AT2G26650

STRING: 3702.AT2G26650.1

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