Abbreviation
Recombinant Rhesus macaque GM-CSF protein (Active)
Purity
>98% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of >1.0x107 IU/mg.
Species
Macaca mulatta (Rhesus macaque)
Expression Region
18-144aa
Target Protein Sequence
APARSPSPGT QPWEHVNAIQ EARRLLNLSR DTAAEMNKTV EVVSEMFDLQ EPSCLQTRLE LYKQGLQGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFQSFKENL KDFLLVIPFD CWEPVQE
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
GM-CSF is a critical hematopoietic cytokine that drives granulocyte and macrophage proliferation, differentiation, and activation through JAK2/STAT5 and MAPK signaling cascades. This tag-free recombinant rhesus macaque GM-CSF, encompassing the full-length mature sequence (18–144 aa), demonstrates exceptional signaling potency with an ED50 below 0.1 ng/ml in TF-1 cell proliferation assays, corresponding to a specific activity exceeding 1.0 × 10⁷ IU/mg — providing a reliable reference standard for cytokine detection assays and a robust positive control for dose-response studies. The species-matched sequence supports use in nonhuman primate in vivo models of inflammation, infection, and tumor biology, while also serving as an effective immunogen or coating antigen for antibody development and validation workflows. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts in sensitive immune cell functional assays, and purity exceeding 98% by SDS-PAGE satisfies the stringent criteria expected for JAK/STAT pathway activation studies and myeloid differentiation experiments.
