Abbreviation
Recombinant Rhesus macaque CSF3 protein, partial (Active)
Purity
>98% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine NFS-60 cells is less than 0.05 ng/ml, corresponding to a specific activity of >2.0x107 IU/mg.
Species
Macaca mulatta (Rhesus macaque)
Expression Region
27-200aa
Target Protein Sequence
TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL RHSLGIPWAP LSSCPSQALQ LTGCLSQLHS SLFLYQGLLQ ALEGISPELS PTLDTLQLDI ADFATTIWQQ MEDLGMAPAL QPTQGAMPAF TSAFQRRAGG VLVASHLQRF LELAYRVLRH LAQS
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Partial of NW_01480557
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
Granulocyte colony-stimulating factor (CSF3) is a key regulator of neutrophil proliferation, differentiation, and survival, making it an essential tool for studying myeloid lineage biology in a nonhuman primate model. This tag-free recombinant rhesus macaque CSF3 (residues 27–200) demonstrates exceptional signaling potency, with an ED50 below 0.05 ng/ml in NFS-60 cell proliferation assays and a specific activity exceeding 2.0 × 10⁷ IU/mg, supporting use in granulopoiesis studies, JAK/STAT and MAPK signaling pathway dissection, and dose–response benchmarking as a reference standard in cytokine detection assays. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts, which satisfies the stringent criteria typical for primary immune cell functional assays and in vivo primate-relevant inflammation or infection models. The protein's >98% purity by SDS-PAGE, combined with its mature sequence fidelity and absence of fusion tags, provides a suitable basis for antibody development workflows including immunization, ELISA coating, and positive control applications.
