Abbreviation
Recombinant Rhesus macaque CCL11 protein (Active)
Purity
>98% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood eosinophils is in a concentration range of 0.1-10.0 ng/ml.
Alternative Names
CCL11; SCYA11Eotaxin; C-C motif chemokine 11; Small-inducible cytokine A11
Species
Macaca mulatta (Rhesus macaque)
Expression Region
24-97aa
Target Protein Sequence
GPDSVATTCC FTLTNKKIPL QRLESYRRII SGKCPQKAVI FKTKLAKDIC ADPKKKWVQD SMKYLDRKSP TPKP
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 6.9.
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CCL11 (eotaxin) is a potent eosinophil-selective chemokine central to allergic inflammation and tissue eosinophilia, making validated recombinant forms essential for mechanistic studies in nonhuman primate models. This tag-free recombinant rhesus macaque CCL11, encompassing the full mature sequence (residues 24–97), drives eosinophil chemotaxis at 0.1–10.0 ng/ml in bioassays using human peripheral blood eosinophils, confirming robust signaling potency suitable for transwell migration assays, CCR3 receptor activation studies, and downstream MAPK or NF-κB pathway analysis. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts in sensitive immune cell assays, supporting use in primary eosinophil functional experiments, in vivo primate inflammation models, and as a reference standard in cytokine detection platforms. Expressed in *E. coli* and purified to greater than 98% by SDS-PAGE, this protein provides a suitable basis for antibody development workflows including immunization, ELISA coating, and positive control applications.
