Abbreviation
Recombinant Rhesus macaque CXCL10 protein (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood T-lymphocytes is in a concentration range of 10- 50 ng/ml.
Alternative Names
CXCL10; SCYB10C-X-C motif chemokine 10; 10 kDa interferon gamma-induced protein; Gamma-IP10; IP-10; Small-inducible cytokine B10
Species
Macaca mulatta (Rhesus macaque)
Expression Region
22-98aa
Target Protein Sequence
IPLSRTVRCT CISISNQPVN PRSLEKLEII PPSQFCPHVE IIATMKKKGE KRCLNPESKA IKNLLKAVSK ERSKRSP
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
0.2 μm filtered PBS, pH 7.4 ,lyophilized
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CXCL10 (IP-10) is a key interferon-γ–inducible chemokine that drives CXCR3-dependent T-lymphocyte recruitment, making it central to studies of antiviral immunity, transplant rejection, and tumor immunology in the rhesus macaque model. This tag-free recombinant protein encompasses the full mature sequence (residues 22–98), preserving native receptor-binding fidelity, and demonstrates confirmed chemotactic activity on human peripheral blood T-lymphocytes at 10–50 ng/ml, which supports use in transwell migration assays, CXCR3 signaling pathway analyses involving MAPK and JAK/STAT activation, and dose–response calibration as a reference standard in IP-10 detection ELISAs. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts in sensitive immune cell functional assays and provide a suitable basis for in vivo inflammation or infection models in non-human primates. Purity exceeding 97% by SDS-PAGE, combined with this stringent endotoxin control, also satisfies the criteria typical for antibody development workflows including immunization, coating antigen use, and Western blot positive controls.
