Abbreviation
Recombinant Rat Cinc3 protein, partial (Active)
Purity
>98% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using rat neutrophils is in a concentration range of 10-100 ng/ml.
Alternative Names
Cxcl2; Cinc3; Mip-2; Mip2; Scyb2C-X-C motif chemokine 2; Cytokine-induced neutrophil chemoattractant 3; CINC-3; Macrophage inflammatory protein 2; MIP2
Species
Rattus norvegicus (Rat)
Expression Region
28-100aa
Target Protein Sequence
VVVASELRCQ CLTTLPRVDF KNIQSLTVTP PGPHCAQTEV IATLKDGHEV CLNPEAPLVQ RIVQKILNKG KAN
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CXCL2 (MIP-2) is a key CXC chemokine that drives neutrophil recruitment during acute inflammation, making validated recombinant forms essential for mechanistic studies of innate immune trafficking. This tag-free rat CXCL2 (residues 28–100), representing the mature secreted sequence, demonstrates confirmed biological activity in a rat neutrophil chemotaxis bioassay at 10–100 ng/ml, providing a suitable basis for Transwell migration assays, CXCR2 signaling pathway studies involving MAPK and NF-κB activation, and in vivo rat models of sterile inflammation or infection. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that can confound neutrophil activation readouts, while purity exceeding 98% by SDS-PAGE supports use as a reliable reference standard in CXCL2 detection ELISAs or as a positive control for antibody validation in western blot. E. coli-derived and untagged, this protein aligns with standards expected in functional immunology research requiring defined, artifact-free chemokine stimulation.
