Abbreviation
Recombinant Mouse Cx3cl1 protein, partial (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human peripheral blood lymphocytes (PBL) is less than 0.5 μg/ml, corresponding to a specific activity of >2000 IU/mg.
Alternative Names
Cx3cl1; Cx3c; Fkn; Scyd1; Fractalkine; FK; C-X3-C motif chemokine 1; CX3C membrane-anchored chemokine; Neurotactin; Small-inducible cytokine D1
Species
Mus musculus (Mouse)
Expression Region
25-100aa
Target Protein Sequence
QHLGMTKCEI MCGKMTSRIP VALLIRYQLN QESCGKRAIV LETTQHRRFC ADPKEKWVQD AMKHLDHQAA ALTKNG
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
Fractalkine (CX3CL1) uniquely functions as both a membrane-anchored adhesion molecule and a soluble chemoattractant, making its chemokine domain critical for studying CX3CR1-dependent leukocyte recruitment. This tag-free recombinant mouse CX3CL1 fragment (25–100 aa) encompasses the mature chemokine domain and demonstrates strong signaling potency, with an ED50 below 0.5 μg/ml in a human PBL proliferation assay corresponding to a specific activity exceeding 2,000 IU/mg — data that supports use in chemotaxis and migration assays, lymphocyte activation studies, and downstream CX3CR1 signaling investigations involving NF-κB and MAPK pathways. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that can confound immune cell functional readouts, making this protein appropriate for in vivo inflammation models and as a reference standard in cytokine detection assays. Purity exceeding 97% by SDS-PAGE, combined with this endotoxin profile, also provides a suitable basis for antibody development, ELISA coating, and Western blot positive controls.
