Abbreviation
Recombinant Mouse Ccl11 protein (Active)
Purity
>96% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using purified eosinophils is in a concentration range of 100-1000 ng/ml.
Alternative Names
Ccl11; Scya11Eotaxin; C-C motif chemokine 11; Eosinophil chemotactic protein; Small-inducible cytokine A11
Species
Mus musculus (Mouse)
Expression Region
24-97aa
Target Protein Sequence
HPGSIPTSCC FIMTSKKIPN TLLKSYKRIT NNRCTLKAIV FKTRLGKEIC ADPKKKWVQD ATKHLDQKLQ TPKP
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CCL11 (Eotaxin) is a potent CC chemokine that selectively recruits eosinophils via CCR3 signaling, making it a critical tool for dissecting allergic inflammation and type 2 immune responses. This tag-free recombinant mouse Eotaxin, expressed in *E. coli* as the full-length mature protein (residues 24–97), demonstrates confirmed biological activity in eosinophil chemotaxis bioassays at 100–1000 ng/ml, supporting use in transwell migration assays, CCR3 receptor activation studies, and MAPK/calcium flux signaling experiments. The faithful mature sequence and absence of fusion tags provide a suitable basis for use as an immunogen in antibody development, as a positive control in ELISA-based cytokine detection, or as a reference standard for quantifying endogenous Eotaxin levels. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts in sensitive primary eosinophil and mast cell assays, while purity exceeding 96% by SDS-PAGE aligns with standards expected in murine models of airway inflammation and allergic disease.
