Abbreviation
Recombinant Mouse Cxcl2 protein (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 1.0-10 ng/ml.
Alternative Names
Cxcl2; Mip-2; Mip2; Scyb2C-X-C motif chemokine 2; Macrophage inflammatory protein 2; MIP2
Species
Mus musculus (Mouse)
Expression Region
28-100aa
Target Protein Sequence
AVVASELRCQ CLKTLPRVDF KNIQSLSVTP PGPHCAQTEV IATLKGGQKVCLDPEAPLVQ KIIQKILNKG KAN
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CXCL2 (MIP-2) is a key neutrophil chemoattractant in murine inflammation, and this tag-free recombinant form encompasses the full mature sequence (residues 28–100), preserving native receptor-binding fidelity for CXCR2-mediated signaling studies. Validated in a human neutrophil chemotaxis bioassay with activity in the 1.0–10 ng/mL range, this protein provides a suitable basis for transwell migration assays, NF-κB and MAPK pathway activation experiments, and in vivo mouse models of acute inflammation or infection where precise neutrophil recruitment kinetics are under investigation. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that would otherwise confound cytokine-dependent readouts in primary immune cell assays, while purity exceeding 97% by SDS-PAGE supports use as a reliable reference standard in CXCL2 detection ELISAs or as a coating antigen for antibody development and validation workflows. Produced in *E. coli*, the recombinant CXCL2 protein aligns with standards expected in quantitative functional studies requiring well-characterized, endotoxin-controlled reagents.
