Abbreviation
Recombinant Mouse Ccl7 protein (Active)
Purity
>95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 100-300 ng/ml.
Alternative Names
Ccl7; Fic; Mcp3; Scya7C-C motif chemokine 7; Intercrine/chemokine MARC; Monocyte chemoattractant protein 3; Monocyte chemotactic protein 3; MCP-3; Protein FIC; Small-inducible cytokine A7
Species
Mus musculus (Mouse)
Expression Region
24-97aa
Target Protein Sequence
QPDGPNASTC CYVKKQKIPK RNLKSYRRIT SSRCPWEAVI FKTKKGMEVC AEAHQKWVEE AIAYLDMKTP TPKP
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
0.2 μm filtered 2xPBS, pH 7.4 ,lyophilized
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CCL7 (MCP-3) is a potent CC chemokine that recruits monocytes, dendritic cells, and lymphocytes through CCR1, CCR2, and CCR3 signaling, making it a critical mediator in inflammatory cell trafficking. This tag-free recombinant mouse CCL7 encompasses the full mature sequence (residues 24–97) and demonstrates confirmed chemotactic activity on human monocytes at 100–300 ng/ml, providing a suitable basis for Transwell migration assays, monocyte recruitment studies, and downstream investigation of MAPK and NF-κB pathway activation. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts in sensitive immune cell functional assays, supporting use in primary monocyte or macrophage activation experiments and in vivo mouse models of inflammation or tumor biology. The protein's >95% purity by SDS-PAGE, combined with its tag-free format, also meets the quality thresholds commonly required for use as a reference standard in cytokine detection assays or as a coating antigen in antibody validation by ELISA.
