Abbreviation
Recombinant Mouse Ccl5 protein (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T lymphocytes is in a concentration range of 1.0-10 ng/ml.
Alternative Names
Ccl5; Scya5C-C motif chemokine 5; MuRantes; SIS-delta; Small-inducible cytokine A5; T-cell-specific protein RANTES
Species
Mus musculus (Mouse)
Expression Region
24-91aa
Target Protein Sequence
SPYGSDTTPC CFAYLSLALP RAHVKEYFYT SSKCSNLAVV FVTRRNRQVC ANPEKKWVQE YINYLEMS
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CCL5 (RANTES) is a key chemotactic cytokine that orchestrates T cell, monocyte, and eosinophil recruitment to sites of inflammation, making it indispensable for studying leukocyte trafficking in immune responses. This tag-free recombinant mouse CCL5, encompassing the full-length mature sequence (residues 24–91), drives chemotaxis of human T lymphocytes at 1.0–10 ng/ml in bioassay, confirming signaling potency suitable for Transwell migration assays, CCR5-dependent MAPK and NF-κB pathway activation studies, and in vivo mouse models of inflammation or tumor immune infiltration. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that commonly confound immune cell functional readouts, which supports use in sensitive T cell activation assays, PBMC chemotaxis experiments, and as a reference standard in cytokine detection platforms. Purity exceeding 97% by SDS-PAGE, combined with this stringent endotoxin control, provides a suitable basis for antibody validation workflows including ELISA coating and Western blot positive controls.
