Abbreviation
Recombinant Mouse Ccl3 protein (Active)
Purity
>98% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemoattract bioassay using murine splenocytes is in a concentration range of 10-100 ng/ml.
Alternative Names
Ccl3; Mip1a; Scya3C-C motif chemokine 3; Heparin-binding chemotaxis protein; L2G25B; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; SIS-alpha; Small-inducible cytokine A3; TY-5
Species
Mus musculus (Mouse)
Expression Region
24-92aa
Target Protein Sequence
APYGADTPTA CCFSYSRKIP RQFIVDYFET SSLCSQPGVI FLTKRNRQIC ADSKETWVQE YITDLELNA
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CCL3 (MIP-1-alpha) is a key inflammatory chemokine that drives monocyte and lymphocyte recruitment, making validated recombinant forms essential for dissecting innate immune signaling. This tag-free recombinant mouse CCL3 encompasses the full mature sequence (residues 24–92) and demonstrates confirmed chemoattractant activity on murine splenocytes at 10–100 ng/ml, providing a suitable basis for Transwell chemotaxis and migration assays, CCR1/CCR5 receptor signaling studies involving MAPK and NF-κB pathway activation, and in vivo murine inflammation or infection models. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that can confound cytokine-dependent readouts in primary immune cell cultures, while purity exceeding 98% by SDS-PAGE supports use as a reliable reference standard in cytokine detection assays or as a positive control for antibody validation by ELISA and Western blot. The E. coli-derived, non-glycosylated format aligns with standards expected in mechanistic studies where glycan-independent bioactivity is preferred.
