Abbreviation
Recombinant Mouse Ccl28 protein, partial (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using murine lymphocytes is in a concentration range of 1.0-10.0 ng/ml.
Alternative Names
Ccl28; Scya28; C-C motif chemokine 28; Small-inducible cytokine A28
Species
Mus musculus (Mouse)
Expression Region
20-130aa
Target Protein Sequence
SEAILPMASS CCTEVSHHVS GRLLERVSSC SIQRADGDCD LAAVILHVKR RRICISPHNR TLKQWMRASE VKKNGRENVC SGKKQPSRKD RKGHTTRKHR TRGTHRHEAS R
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CCL28 plays a critical role in mucosal immunity by directing lymphocyte homing to epithelial tissues, making it a key target for studying gut- and lung-associated immune responses. This tag-free recombinant mouse CCL28 (residues 20–130) encompasses the mature protein sequence and demonstrates potent chemotactic activity toward murine lymphocytes at 1.0–10.0 ng/ml, providing a suitable basis for Transwell migration assays, mucosal inflammation models, and signaling pathway studies involving CCR3/CCR10 receptor activation. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts in sensitive immune cell assays, which satisfies the purity and endotoxin criteria typical for in vivo studies of mucosal trafficking and ex vivo lymphocyte functional assays. Produced in *E. coli* with greater than 97% purity by SDS-PAGE, this protein also supports use as a reference standard in chemokine detection assays or as a coating antigen for antibody validation by ELISA.
