Abbreviation
Recombinant Mouse Ccl21c protein (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using murine T-lymphocytes is in a concentration range of 10-100 ng/ml.
Alternative Names
Ccl21c; Scya21cC-C motif chemokine 21c; 6Ckine; Beta-chemokine exodus-2; Small-inducible cytokine A21c; Thymus-derived chemotactic agent 4; TCA4
Species
Mus musculus (Mouse)
Expression Region
24-133aa
Target Protein Sequence
SDGGGQDCCL KYSQKKIPYS IVRGYRKQEP SLGCPIPAIL FLPRKHSKPE LCANPEEGWV QNLMRRLDQP PAPGKQSPGC RKNRGTSKSG KKGKGSKGCK RTEQTQPSRG
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CCL21c (TCA4/6Ckine) is a homeostatic chemokine critical for guiding T cells and dendritic cells to secondary lymphoid organs via CCR7 signaling, making it indispensable in studies of adaptive immune trafficking. This tag-free recombinant mouse CCL21c, encompassing the full mature sequence (24–133 aa), drives chemotaxis of murine T-lymphocytes at 10–100 ng/ml in bioassay, providing a suitable basis for Transwell migration assays, CCR7 receptor activation studies, and MAPK or PI3K pathway dissection downstream of chemokine engagement. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that can confound immune cell readouts, supporting use in sensitive in vivo lymphocyte homing models and primary cell-based functional assays where cytokine-specific effects must be cleanly resolved. Purity exceeding 97% by SDS-PAGE further qualifies this protein as a reliable reference standard in chemokine detection assays and as a positive control for anti-CCL21c antibody validation by ELISA or Western blot.
