Abbreviation
Recombinant Mouse Ccl19 protein (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human mature dendritic cells is in a concentration range of 10-100 ng/ml.
Alternative Names
Ccl19; Elc; Scya19; C-C motif chemokine 19; Epstein-Barr virus-induced molecule 1 ligand chemokine; EBI1 ligand chemokine; ELC; Small-inducible cytokine A19
Species
Mus musculus (Mouse)
Expression Region
26-108aa
Target Protein Sequence
GANDAEDCCL SVTQRPIPGN IVKAFRYLLN EDGCRVPAVV FTTLRGYQLC APPDQPWVDR IIRRLKKSSA KNKGNSTRRS PVS
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CCL19 (ELC) plays a central role in directing dendritic cell and lymphocyte trafficking to secondary lymphoid organs through CCR7 engagement, making it indispensable for studying adaptive immune initiation. This tag-free recombinant mouse CCL19, encompassing the full mature sequence (residues 26–108), drives chemotaxis of human mature dendritic cells at 10–100 ng/ml, confirming signaling potency suitable for transwell migration assays, CCR7-dependent pathway studies, and in vivo lymphocyte homing models. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that commonly confound dendritic cell and T-cell functional readouts, supporting use in sensitive cell-based activation, differentiation, and signaling experiments interrogating MAPK or NF-κB cascades downstream of CCR7. Purity exceeding 97% by SDS-PAGE, combined with this stringent endotoxin control, also provides a suitable basis for deployment as a reference standard in chemokine detection assays or as a positive control antigen in ELISA and antibody validation workflows.
