Abbreviation
Recombinant Human CXCL17 protein (Active)
Purity
>96% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by its ability to induce VEGF expression using murine endothelial cells is less than 5.0 μg/ml, corresponding to a specific activity of >200 IU/mg.
Alternative Names
CXCL17; VCC1; UNQ473/PRO842C-X-C motif chemokine 17; 6-Cys CXCL17; Dendritic cell and monocyte chemokine-like protein; DMC; VEGF coregulated chemokine 1) [Cleaved into: 4-Cys CXCL17]
Species
Homo sapiens (Human)
Expression Region
22-119aa
Target Protein Sequence
SSLNPGVARG HRDRGQASRR WLQEGGQECE CKDWFLRAPR RKFMTVSGLP KKQCPCDHFK GNVKKTRHQR HHRKPNKHSR ACQQFLKQCQ LRSFALPL
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 3 % Trehalose.
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CXCL17, also known as VEGF coregulated chemokine 1, plays a distinctive role in mucosal immunity and angiogenesis-linked chemotaxis, making it a valuable tool for dissecting crosstalk between immune cell recruitment and vascular signaling. This tag-free recombinant human CXCL17 encompasses the full-length mature sequence (22–119 aa) expressed in *E. coli* and demonstrates an ED50 of less than 5.0 μg/ml for inducing VEGF expression in murine endothelial cells, corresponding to a specific activity exceeding 200 IU/mg — data that supports use in chemotaxis and migration assays, VEGF-dependent signaling pathway studies, and cell-based functional assays examining endothelial activation or macrophage recruitment. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts in sensitive immune cell experiments and in vivo inflammation models, while purity above 96% by SDS-PAGE provides a suitable basis for deployment as a reference standard in cytokine detection assays or as a reliable antigen for antibody development and ELISA validation.
