Abbreviation
Recombinant Human FAM19A2 protein (Active)
Purity
>95% as determined by SDSPAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity is determined by its ability to enhance neurite outgrowth of E16?E18 rat embryonic cortical neurons. rHuTAFA?2, immobilized at 6?24 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.
Alternative Names
Chemokine like protein TAFA 2; Chemokine-like protein TAFA-2; Chemokine-like protein TAFA2; DKFZp761E1217; DKFZp781P0552; F19A2_HUMAN; FAM19A2; family with sequence similarity 19 (chemokine (C-C motif)-like); member A2; MGC42403; Protein FAM19A2; TAFA 2; TAFA2
Species
Homo sapiens (Human)
Expression Region
31-131aa
Target Protein Sequence
ANHHKAHHVK TGTCEVVALH RCCNKNKIEE RSQTVKCSCF PGQVAGTTRA APSCVDASIV EQKWWCHMQP CLEGEECKVL PDRKGWSCSS GNKVKTTRVT H
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
FAM19A2 (TAFA-2) is a brain-enriched chemokine-like cytokine implicated in neuroimmune crosstalk, and this tag-free recombinant form—covering the full mature sequence (residues 31–131)—preserves native folding critical for receptor engagement. Validated biological activity confirms that the protein significantly enhances neurite outgrowth in E16–E18 rat embryonic cortical neurons when immobilized at 6–24 μg/mL, providing a suitable basis for cell-based functional assays investigating neuronal differentiation, MAPK or other downstream signaling pathway activation, and chemotaxis studies exploring TAFA-2-mediated immune cell migration. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts, which aligns with standards expected in primary neuron cultures, in vivo neuroimmune models, and sensitive immune cell activation experiments. Purity exceeding 95% by SDS-PAGE, combined with this stringent endotoxin control, also supports use as a coating antigen for antibody development or as a reliable positive control in ELISA-based cytokine detection assays.
