Abbreviation
Recombinant Human CXCL6 protein, partial (Active)
Purity
>98% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 10-50 ng/ml.
Alternative Names
C X C motif chemokine 6; Chemokine (C X C motif) ligand 6; Chemokine alpha 3; CKA 3; CKA-3; CKA3; CXCL 6; CXCL6; CXCL6_HUMAN; GCP 2; GCP-2; Granulocyte chemotactic protein 2; N-processed variant 3; SCYB6; Small inducible cytokine B6; Small inducible cytokine subfamily B (Cys X Cys) member 6; Small inducible cytokine subfamily B (Cys X Cys) member b; Small-inducible cytokine B6
Species
Homo sapiens (Human)
Expression Region
43-114aa
Target Protein Sequence
VLTELRCTCL RVTLRVNPKT IGKLQVFPAG PQCSKVEVVA SLKNGKQVCL DPEAPFLKKV IQKILDSGNK KN
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CXCL6 (GCP-2) is a potent ELR+ CXC chemokine that signals through CXCR1 and CXCR2 to drive neutrophil recruitment, making it a critical tool for dissecting innate immune trafficking mechanisms. This tag-free recombinant human CXCL6, spanning residues 43–114 of the mature processed sequence, demonstrates robust chemotactic activity on primary human neutrophils at 10–50 ng/ml, providing a suitable basis for Transwell migration assays, CXCR1/CXCR2 signaling pathway studies involving MAPK and NF-κB activation, and in vivo neutrophilic inflammation models. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that can confound neutrophil priming and cytokine release readouts, which satisfies the stringent criteria typical for primary immune cell functional assays. Produced in *E. coli* with greater than 98% purity by SDS-PAGE, this recombinant CXCL6 protein also supports use as a reference standard in chemokine detection assays and as a coating antigen for anti-CXCL6 antibody validation by ELISA.
