Abbreviation
Recombinant Human CXCL13 protein (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human B cells is in a concentration range of 1.0-10 ng/ml.
Alternative Names
ANGIE; ANGIE2 ; B cell attracting chemokine 1; B cell-attracting chemokine 1; B lymphocyte chemoattractant; B-cell chemoattractant ; B-cell-attracting chemokine 1; B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1); BCA-1; BLC; BLR1L ; C-X-C motif chemokine 13; Chemokine (C-X-C motif) ligand 13 ; Chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant) ; Chemokine; CXC motif; ligand 13; CXC chemokine BLC; CXCL13; CXL13_HUMAN; SCYB13; Small inducible cytokine B subfamily (Cys-X-Cys motif); member 13 (B-cell chemoattractant); Small inducible cytokine B13; Small inducible cytokine subfamily B; member 13; Small-inducible cytokine B13
Species
Homo sapiens (Human)
Expression Region
23-109aa
Target Protein Sequence
VLEVYYTSLR CRCVQESSVF IPRRFIDRIQ ILPRGNGCPR KEIIVWKKNK SIVCVDPQAE WIQRMMEVLR KRSSSTLPVP VFKRKIP
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CXCL13 (BLC) is a critical B cell chemoattractant that orchestrates lymphocyte trafficking to secondary lymphoid organs, making it a key target in studies of germinal center formation, autoimmune pathology, and lymphoma biology. This tag-free recombinant human CXCL13, encompassing the full-length mature sequence (residues 23–109), drives chemotaxis of primary human B cells at 1.0–10 ng/ml, providing a suitable basis for Transwell migration assays, CXCR5 signaling pathway studies, and in vivo models of inflammation or tumor immune microenvironment remodeling. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that can confound immune cell activation readouts, satisfying the purity and endotoxin criteria typical for sensitive B cell functional assays, antibody validation workflows, and use as a reference standard in CXCL13 detection ELISAs. Produced in *E. coli* with greater than 97% purity by SDS-PAGE, this recombinant CXCL13 protein supports reliable dose–response characterization in chemotaxis and CXCR5-dependent MAPK or NF-κB activation experiments.
