Abbreviation
Recombinant Human CCL4 protein (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human CCR5 transfected murine BaF3 cells is less than 2.0 ng/ml, corresponding to a specific activity of >5.0x105 IU/mg.
Alternative Names
CCL4L1; CCL4L; LAG1; SCYA4L1;; CCL4L2; CCL4L; SCYA4L2C-C motif chemokine 4-like; Lymphocyte activation gene 1 protein; LAG-1; Macrophage inflammatory protein 1-beta; MIP-1-beta; Monocyte adherence-induced protein 5-alpha; Small-inducible cytokine A4-like
Species
Homo sapiens (Human)
Expression Region
24-92aa
Target Protein Sequence
APMGSDPPTA CCFSYTARKL PRNFVVDYYE TSSLCSQPAV VFQTKRGKQV CADPSESWVQ EYVYDLELN
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
0.2 μm filtered PBS, pH 7.4 ,lyophilized
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CCL4L1 is a potent CCR5 ligand that plays a key role in leukocyte recruitment during inflammatory and antiviral immune responses, making it a valuable tool for dissecting chemokine-driven signaling. This tag-free recombinant protein encompasses the full-length mature sequence (residues 24–92) and demonstrates strong biological activity, with an ED50 below 2.0 ng/ml in a CCR5-transfected BaF3 cell proliferation assay, corresponding to a specific activity exceeding 5.0 × 10⁵ IU/mg — potency that supports use in chemotaxis and migration assays, CCR5-mediated signaling studies involving MAPK and NF-κB pathways, and in vivo inflammation or infection models. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts in sensitive immune cell experiments, while purity greater than 97% by SDS-PAGE provides a suitable basis for deployment as a reference standard in cytokine detection assays or as a positive control for antibody validation by ELISA and Western blot.
