Abbreviation
Recombinant Human CCL3L1 protein (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/ml.
Alternative Names
464.2; C-C motif chemokine 3-like 1; CCL3L3; CL3L1_HUMAN; D17S1718; G0/G1 switch regulatory protein 19-2; G0S19-2; LD78; LD78-beta(1-70); LD78-beta(5-70); LD78BETA; PAT 464.2; SCYA3L; SCYA3L1; Small-inducible cytokine A3-like 1; Tonsillar lymphocyte LD78 beta protein
Species
Homo sapiens (Human)
Expression Region
24-93aa
Target Protein Sequence
APLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPSV IFLTKRGRQV CADPSEEWVQ KYVSDLELSA
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CCL3L1 is a potent CCR5 ligand whose copy-number variation has been linked to differential HIV-1 susceptibility, making precise functional studies of this chemokine critically important. This tag-free recombinant human CCL3L1 encompasses the full-length mature sequence (residues 24–93) and demonstrates robust biological activity in a human monocyte chemotaxis bioassay at 1.0–10 ng/mL, providing a suitable basis for migration assays, CCR5/CCR1 signaling pathway analyses involving MAPK and NF-κB activation, and in vivo inflammation models. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that can confound monocyte and macrophage functional readouts, while >97% purity by SDS-PAGE supports use as a reliable reference standard in cytokine detection assays or as a positive control for antibody validation by ELISA and western blot. The E. coli-expressed protein aligns with standards expected in cell-based activation and differentiation experiments where attribution of response to the target chemokine is essential.
