Abbreviation
Recombinant Human CCL23 protein, partial (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration of 10-50 ng/ml.
Alternative Names
C C motif chemokine ligand 23; C-C motif chemokine 23; C6 beta chemokine ; CCL 23; CCL23; CCL23(30-99); CCL23_HUMAN; Chemokine (C C motif) ligand 23; Chemokine CC motif ligand 23; ck beta 8; CK-beta-8; CKB 8 ; CKB-8; Ckb-8-1; CKB8 ; Hmrp-2a; Macrophage inflammatory protein 3; MIP 3; MIP-3; MIP3; MPIF 1; MPIF-1; MPIF1; Myeloid progenitor inhibitory factor 1; OTTHUMP00000163948 ; SCYA 23; SCYA23; Small inducible cytokine A 23; Small inducible cytokine subfamily A (Cys-Cys); member 23 ; Small inducible cytokine subfamily A; member 23; Small-inducible cytokine A23
Species
Homo sapiens (Human)
Target Protein Sequence
RVTKDAETEF MMSKLPLENP VLLDRFHATS ADCCISYTPR SIPCSLLESY FETNSECSKP GVIFLTKKGR RFCANPSDKQ VQVCMRMLKL DTRIKTRKN
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Partaial of NM_145898
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CCL23 (MPIF-1) plays a dual role in myeloid progenitor regulation and chemotactic recruitment, making it a valuable tool for dissecting innate immune signaling networks. This tag-free recombinant human CCL23 (residues 22–120) encompasses the mature protein sequence and demonstrates confirmed chemotactic activity on human T-lymphocytes at 10–50 ng/ml, supporting use in Transwell migration assays, GPCR-mediated signaling studies involving MAPK and NF-κB pathways, and functional screening during antibody development campaigns. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that can confound cytokine-dependent readouts in primary immune cell cultures, providing a suitable basis for in vivo inflammation models and sensitive cell-based activation or differentiation assays. Purity exceeding 97% by SDS-PAGE, combined with this stringent endotoxin control, also satisfies the criteria typical for use as a reference standard in chemokine detection ELISAs and Western blot positive controls.
