Abbreviation
Recombinant Human CCL21 protein (Active)
Purity
>95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human lymphocytes is in a concentration range of 10-100 ng/ml.
Alternative Names
6Ckine; Beta chemokine exodus 2; Beta-chemokine exodus-2; C C motif chemokine ligand 21; C-C motif chemokine 21; CCL21; CCL21_HUMAN; Chemokine (C-C motif) ligand 21; Chemokine CC motif ligand 21; CKb9; ECL; Efficient Chemoattractant for Lymphocytes; SCYA21; Secondary lymphoid tissue chemokine; Secondary lymphoid-tissue chemokine; SLC; Small inducible cytokine A21 ; Small inducible cytokine subfamily A (Cys-Cys); member 21; Small-inducible cytokine A21; TCA4; UNQ784/PRO1600
Species
Homo sapiens (Human)
Expression Region
24-134aa
Target Protein Sequence
SDGGAQDCCL KYSQRKIPAK VVRSYRKQEP SLGCSIPAIL FLPRKRSQAE LCADPKELWV QQLMQHLDKT PSPQKPAQGC RKDRGASKTG KKGKGSKGCK RTERSQTPKG P
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered concentrated solution in 2 × PBS, pH 7.0.
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CCL21 directs the homing of T cells and dendritic cells to secondary lymphoid organs through CCR7 engagement, making it a critical tool for dissecting lymphocyte trafficking mechanisms. This tag-free recombinant human CCL21, encompassing the full-length mature sequence (24–134aa), demonstrates confirmed chemotactic activity on human lymphocytes at 10–100 ng/ml, providing a suitable basis for chemotaxis and transwell migration assays, CCR7 signaling pathway studies, and in vivo models of lymphocyte homing or tumor immunology. The protein's endotoxin level of less than 1.0 EU/μg minimizes LPS-driven artifacts that can confound immune cell functional readouts, satisfying the purity and endotoxin criteria typical for sensitive primary lymphocyte activation and dendritic cell maturation experiments. Produced in *E. coli* with greater than 95% purity by SDS-PAGE, this preparation also supports use as a reference standard in chemokine detection assays or as a coating antigen for anti-CCL21 antibody validation by ELISA.
