Abbreviation
Recombinant Human CCL20 protein (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-50 ng/ml.
Alternative Names
Beta chemokine exodus 1; Beta-chemokine exodus-1; C C motif chemokine ligand 20; C-C motif chemokine 20; CC chemokine LARC; Ccl20; CCL20(2-70); CCL20_HUMAN; Chemokine (C C motif) ligand 20; Chemokine C-C motif chemokine 20; Chemokine CC motif ligand 20; CKb4; Exodus 1; Exodus; LARC; Liver and activation regulated chemokine; Liver and activation-regulated chemokine; Macrophage inflammatory protein 3 alpha; MIP 3 alpha; MIP 3A; MIP-3-alpha; MIP-3a; MIP3 alpha; MIP3A; SCYA20; Small inducible cytokine A20 ; Small inducible cytokine subfamily A (Cys Cys) member 20; Small-inducible cytokine A20; ST38
Species
Homo sapiens (Human)
Expression Region
27-96aa
Target Protein Sequence
ASNFDCCLGY TDRILHPKFI VGFTRQLANE GCDINAIIFH TKKKLSVCAN PKQTWVKYIV RLLSKKVKNM
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CCL20 drives chemotaxis of CCR6-expressing lymphocytes and dendritic cells, making it a critical tool for dissecting mucosal immunity and inflammatory cell trafficking. This tag-free recombinant human CCL20, encompassing the full mature sequence (27–96 aa), induces robust T-lymphocyte migration at 10–50 ng/ml in chemotaxis bioassays, confirming signaling potency through the CCR6/CCL20 axis suitable for Transwell migration assays, NF-κB and MAPK pathway activation studies, and in vivo models of mucosal inflammation or tumor immune infiltration. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that can confound cytokine-dependent readouts in primary immune cell cultures, supporting use in sensitive dendritic cell differentiation experiments and PBMC-based functional assays. Purity exceeding 97% by SDS-PAGE, combined with this stringent endotoxin control, also provides a suitable basis for deployment as an ELISA reference standard or a positive control in antibody validation workflows.
