Abbreviation
Recombinant Human CCL18 protein (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration of 1.0-10 ng/ml.
Alternative Names
Alternative macrophage activation associated CC chemokine 1; Alternative macrophage activation-associated CC chemokine 1; AMAC-1; AMAC1; CC chemokine PARC; CCL18; CCL18(4-69); CCL18_HUMAN; CKb7; DC-CK1; DCCK1 ; DCCK1; Dendritic cell chemokine 1; Macrophage inflammatory protein 4; MIP-4; MIP4; PARC; Pulmonary and activation regulated chemokine; Pulmonary and activation-regulated chemokine; SCYA18; Small inducible cytokine A18 ; Small inducible cytokine A18; Small-inducible cytokine A18
Species
Homo sapiens (Human)
Expression Region
21-89aa
Target Protein Sequence
AQVGTNKELC CLVYTSWQIP QKFIVDYSET SPQCPKPGVI LLTKRGRQIC ADPNKKWVQK YISDLKLNA
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CCL18 plays a distinctive role in adaptive immunity by selectively recruiting naïve T cells and dendritic cells to sites of antigen presentation, making it a key tool for dissecting chemokine-driven immune cell trafficking. This tag-free recombinant protein encompasses the full-length mature sequence (residues 21–89) and demonstrates robust chemotactic activity on human T-lymphocytes at 1.0–10 ng/mL, providing a suitable basis for Transwell migration assays, dendritic cell co-culture experiments, and in vivo models of inflammation or tumor immune infiltration. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts in sensitive immune cell assays, which supports use in signaling pathway studies examining MAPK or NF-κB activation downstream of CCR8 engagement. Purity exceeding 97% by SDS-PAGE, combined with this stringent endotoxin control, also aligns with standards expected in antibody validation workflows and as a reference standard in CCL18 detection ELISAs.
