Abbreviation
Recombinant Human CCL14 protein, partial (Active)
Purity
>96% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 5.0-20 ng/ml.
Alternative Names
CC-1; CC-3; CCL14; CCL14_HUMAN; chemokine (C-C motif) ligand 14; Chemokine CC-1/CC-3; chemokine CC1; chemokine CC3; chemokine HCC1; chemokine HCC3; CKb1; HCC 1; HCC 3; HCC-1(1-74); HCC-1(9-74); HCC-1/HCC-3; HCC-3; HCC1; HEMOFILTRATE CC CHEMOKINE 1; MCIF; NCC-2; NCC2; new CC chemokine 2; SCYA14; SCYL2; small inducible cytokine subfamily A (Cys-Cys); member 14; Small-inducible cytokine A14; SY14
Species
Homo sapiens (Human)
Expression Region
22-93aa
Target Protein Sequence
TESSSRGPYH PSECCFTYTT YKIPRQRIMD YYETNSQCSK PGIVFITKRG HSVCTNPSDK WVQDYIKDMK EN
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CCL14 (HCC-1) is among the most abundant chemokines in human plasma, yet its truncated active form (residues 22–93) drives potent monocyte chemotaxis that the full-length protein lacks, making sequence fidelity of this processed variant critical for meaningful functional data. This tag-free recombinant human CCL14 elicits monocyte migration at 5–20 ng/ml in chemotaxis bioassays, providing a suitable basis for Transwell migration studies, CCR1/CCR5 receptor activation experiments, and downstream MAPK or NF-κB signaling pathway analyses. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that commonly confound monocyte and macrophage functional readouts, supporting use in primary immune cell assays and in vivo inflammation models where spurious innate immune activation would compromise interpretation. Purity exceeding 96% by SDS-PAGE, combined with this stringent endotoxin profile, also satisfies the criteria typical for ELISA standard curves and antibody validation workflows requiring a reliable positive control.
