Abbreviation
Recombinant Human CCL1 protein, partial (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-100 ng/ml.
Alternative Names
C-C motif chemokine 1; Ccl1; CCL1_HUMAN; Chemokine CC Motif Ligand 1; inflammatory cytokine I-309; P500; SCYA1; SISe; small inducible cytokine A1; Small-inducible cytokine A1; T lymphocyte secreted protein I-309; T lymphocyte secreted protein I-309; T lymphocyte-secreted protein I-309; TCA3
Species
Homo sapiens (Human)
Expression Region
23-96aa
Target Protein Sequence
SKSMQVPFSR CCFSFAEQEI PLRAILCYRN TSSICSNEGL IFKLKRGKEA CALDTVGWVQ RHRKMLRHCP SKRK
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
CCL1 (also known as I-309) plays a critical role in recruiting CCR8-expressing T cells and monocytes to sites of inflammation, making it a key target in studies of Th2-mediated immunity and tumor immune evasion. This tag-free recombinant human CCL1 encompasses the mature protein sequence (residues 23–96) and demonstrates confirmed chemotactic activity on primary human T-lymphocytes at 10–100 ng/ml, providing a suitable basis for Transwell migration assays, CCR8 signaling pathway analyses involving MAPK and NF-κB activation, and in vivo models of inflammatory cell trafficking. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that can confound immune cell functional readouts, while purity exceeding 97% by SDS-PAGE supports use as a reliable reference standard in CCL1 detection assays or as a coating antigen for anti-CCL1 antibody validation by ELISA. The E. coli-derived, non-glycosylated format ensures batch-to-batch consistency for reproducible dose-response experiments.
