SKP2

SKP2, also known as S-phase kinase-associated protein 2, has alternative names including p45, FBXW2, and SKP2. SKP2 is a component of the E3 ubiquitin ligase SCF complex, comprising an F-box sequence, linker sequence, C-terminal region, and ten leucine-rich repeat (LRR) domains, where the LRRs play a crucial role in substrate recognition. SKP2 can induce ubiquitination at the K48 residue, leading to proteasome-mediated hydrolysis of labeled substrates, affecting the stability of various cell cycle-related proteins such as p27, FOXO1, and CARM1. By controlling the stability of cell cycle-related proteins, SKP2 participates in cell cycle regulation, proliferation, migration, and tumor development, with its expression levels closely related to the occurrence, progression, and prognosis of multiple tumors.

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

SKP2 Recombinant Monoclonal Antibody(CSB-RA155372A0HU)

Validated Data

Immunofluorescence staining of HepG2 Cells with CSB-RA155372A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Overlay histogram showing Hela cells stained with CSB-RA155372A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*10⁶ cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1µg/1*10⁶ cells) used under the same conditions. Acquisition of >10,000 events was performed.

The following SKP2 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

SKP2 Antibodies

SKP2 Proteins

SKP2 Antibodies

SKP2 Antibodies for Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Code	Product Name	Species Reactivity	Application
CSB-PA331655XA01SVG	SKP2 Antibody	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	ELISA, WB (ensure identification of antigen)

SKP2 Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA021362GA01HU	SKP2 Antibody	Human,Mouse,Rat	ELISA,WB
CSB-PA613392LA01HU	SKP2 Antibody	Human	ELISA, WB, IHC
CSB-PA613392LD01HU	SKP2 Antibody, Biotin conjugated	Human	ELISA
CSB-PA004098	SKP2 Antibody	Human	WB, IHC, IF, ELISA
CSB-RA155372A0HU	SKP2 Recombinant Monoclonal Antibody	Human	ELISA, IF, FC
CSB-RA224072A0HU	SKP2 Recombinant Monoclonal Antibody	Human	ELISA, IHC, IF

SKP2 Proteins

SKP2 Proteins for Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Code	Product Name	Source
CSB-YP331655SVG CSB-EP331655SVG CSB-BP331655SVG CSB-MP331655SVG CSB-EP331655SVG-B	Recombinant Saccharomyces cerevisiae F-box protein SKP2 (SKP2), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

SKP2 Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-EP613392HU	Recombinant Human S-phase kinase-associated protein 2 (SKP2)	E.coli
CSB-YP613392HU CSB-EP613392HU CSB-BP613392HU CSB-MP613392HU CSB-EP613392HU-B	Recombinant Human S-phase kinase-associated protein 2 (SKP2)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-EP613392HUc7	Recombinant Human S-phase kinase-associated protein 2 (SKP2)	E.coli

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SKP2

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