FOXA1, a pivotal transcription factor, is essential for guiding key biological processes like organ development, cellular differentiation, and metabolic regulation. This factor engages with specific DNA sequences to initiate gene transcription and reshape chromatin structure, subsequently affecting the function of other transcription factors. In cancer, FOXA1's collaboration with hormone receptors such as ER and AR is fundamental in controlling cancer cell activities, including growth, spread, and resistance to treatment. Mutations or epigenetic alterations in FOXA1 are associated with the development and resistance to therapy in various cancers, especially those positive for estrogen receptors. FOXA1 also contributes to the control of metabolic pathways, which affects cellular energy balance. As a result, FOXA1 is not only crucial for normal cell function but also a critical factor in disease progression, making it a significant target for cancer treatment strategies.
Popular
Product:
FOXA1 Recombinant Monoclonal Antibody(CSB-RA246102A0HU)
Validated Data
Western Blot
Positive WB detected in: MCF-7 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, Hela whole cell lysate
All lanes: FOXA1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 50, 46 kDa
Observed band size: 50 kDa
IHC image of CSB-RA246102A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA246102A0HU diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunoprecipitating FOXA1 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-RA246102A0HU in Hela whole cell lysate.
For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA246102A0HU(2µg)+ Hela whole cell lysate(500µg)
Lane 3: Hela whole cell lysate (10µg)
The following FOXA1
reagents supplied by CUSABIO are manufactured under a
strict quality control system. Multiple applications
have been validated and solid technical support is
offered.
