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TBST Buffer

TBST Buffer
Stocks solutions
  • A: Tris (C4H11NO3 mw: 121.14 g/mol)
  • B: Hydrochloric Acid (HCl mw: 36.46 g/mol)
  • C: Sodium Chloride (NaCl mw: 58.44 g/mol)
  • M: Tween20 (C58H114O26 mw: 1227.5 g/mol)
  • N: Distilled water
To prepareL of TBST Buffer ( ×, pH: 7.5 )

Input buffer volume, pH, molar concentration to get formula.

Table 1. Required components

Component Mass（g）
A (Tris, mw: 121.14 g/mol) A
B (Hydrochloric Acid, mw: 36.46 g/mol) B
C (Sodium Chloride, mw: 58.44 g/mol) C
M (Tween20, mw: 1227.5 g/mol) M
Steps：
  • Prepare N mL of distilled water in a suitable container.
  • Add A g of Tris to the solution.
  • Add B g of Hydrochloric Acid to the solution.
  • Add C g of Sodium Chloride to the solution.
  • Add M g of Tween20 to the solution.
  • Adjust solution to final desired pH using HCl or NaOH
  • Add distilled water until volume is X L.
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