PBS Buffer


PBS Buffer
Stocks solutions
  • A: Disodium Hydrogen Phosphate (Na2HPO4 mw: 141.96 g/mol)
  • B: Sodium Dihydrogen Phosphate (NaH2PO4 mw: 119.98 g/mol)
  • C: Sodium Chloride (NaCl mw: 58.44 g/mol)
  • D: Distilled water
To prepareL of PBS Buffer ( pH )

Input buffer volume, pH, molar concentration to get formula.

Table 1. Required components

Component Mass(g)
A (Disodium Hydrogen Phosphate, mw: 141.96 g/mol) A
B (Sodium Dihydrogen Phosphate, mw: 119.98 g/mol)) B
C (Sodium Chloride, mw: 58.44 g/mol) C
Steps:
  • Prepare D mL of distilled water in a suitable container.
  • Add A g of Disodium Hydrogen Phosphate to the solution.
  • Add B g of Sodium Dihydrogen Phosphate to the solution.
  • Add C g of Sodium Chloride to the solution.
  • Adjust solution to final desired pH using HCl or NaOH
  • Add distilled water until volume is X L.
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