HEPES Buffer


HEPES Buffer
Stocks solutions
  • A: HEPES (C8H18N2O4S mw: 238.3 g/mol)
  • B: Distilled water
To prepareL of HEPES Buffer ( M, pH 6.8~8.2 )

Input buffer volume, pH, molar concentration to get formula.

Table 1. Required components

Component Mass(g)
A (HEPES, mw: 238.3 g/mol) A
Steps:
  • Prepare C mL of distilled water in a suitable container.
  • Add A g of HEPES to the solution.
  • Adjust solution to final desired pH using HCl or NaOH
  • Add distilled water until volume is X L.
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