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Yeast Expression Service

Yeast Expression Service

Yeast protein expression system is the most economical and efficient
eukaryotic expression system for both secretion and intracellular expression.
And the medium is relatively inexpensive for large-scale production.And the conditions for yeast cultivate are not strict,so It is ideally suitable for large-scale production of recombinant eukaryotic proteins.
The protein expressed by the mammalian cell expression system which is high-quality is very close to the natural protein.But the Low expression levels, the high cost of medium and the culture conditions relatively fastidious restrict the promotion of the use of mammalian cell expression system used to produce
high-quality protein material. as well as mammalian cell expression system, Yeast protein expression systems serve as a eukaryotic expression system integrate the advantages of the mammalian cell expression system.So the protein expressed by yeast protein expression system could be modificated such as glycosylation, acylation, Phosphorylation and so on to ensure that the protein native conformation.So It could Used to produce protein material with high added value that is very close to natural protein. The facts proved that our protein produced by Yeast protein expression system which served as raw materials for downstream preparation monoclonal antibody had good properties of the recognition of natural protein through the feedback of the customs.
our efficient seed screening work can make your order to obtain the highest yield in the shortest time

Yeast protein expression system economical menu service
Milestones	Specifications	Deliverables	Timeline
1. protokaryon cloning	Genes obtain	Nucleic acid electrophoresis pictures	2 weeks
	PCR amplification product connected to Ppic9k modified by our company Transformate to DH5 α
	Large-scale synthesis of recombinant plasmid
	Linearize the recombinant plasmid
2. Expression optimization	Electric Transformation of the appropriate yeast host strain	Nucleic acid electrophoresis pictures	2-3 weeks
2. Expression optimization	PCR verification of 10 strains randomly selected from transformation plate	Nucleic acid electrophoresis pictures	2-3 weeks
3. Protein expression and purification	randomly select a PCR verification positive strain for 1 L large-scale culture	Expressed supernatant SDS-PAGE	1-2 weeks
	The dispose of the supernatant and One-step purification	Purified product SDS-PAGE and western blot
	Purified product SDS-PAGE and western blot	1L supernatant Purified product

Yeast protein expression system service standard menu
Milestones	Specifications	Deliverables	Timeline
1. protokaryon cloning	Genes obtain	Nucleic acid electrophoresis pictures	2 weeks
	PCR amplification product connected to Ppic9k modified by our company，Transformate to DH5 α
	Linearize the recombinant plasmid
	Linearize the recombinant plasmid
2. Expression optimization	Electric Transformation of the appropriate yeast host strain	Nucleic acid electrophoresis pictures	5-6 weeks
	The poly copies screening via genetic G418
	PCR identification of multi-copy cloning
	Select more than 40 multi-copies strains for small-scale expression	Purified product SDS-PAGE and western blot
3. Protein expression and purification	Select the best strain for large-scale expression according to the customer request	Expressed supernatant SDS-PAGE <

Order Information

Email:tech@cusabio.com

Tele:+86-27-87582341

Fax:+86-27-87196150

Manual:Eukaryotic expression

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