Sumo tag Monoclonal Antibody

Code: CSB-MA000132M0m

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Size:

50μg100μg

US$120

Quantity:

Species Reactivity: N/A

Raised in: mouse

Application: ELISA,WB

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Product Details

Raised in

mouse

Species Reactivity

N/A

Immunogen

sumo tag protein

Conjugate

Non-conjugated

Isotype

IgG1

Clone No.

1A52F8

Buffer

Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Concentration

It differs from different batches. Please contact us to confirm it.

Form

liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA,WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

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WB:SUMO-tagged fusion protein(20ng) was subjected to SDS-PAGE followed by Western Blot with CSB-MA000132M0m at dilution of
Lane 1:1000, Lane 2:2000, Lane 3:4000
Lane 4:8000, Lane 5:16000, Lane 6:32000
Lane 7:64000, Lane 8:128000, Lane 9:256000
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size:55kd
Observed band size:55kd

Description

The mice spleens isolated from sumo tag-immunized mice are fused with myeloma cells to generate hybridomas. Hybridoma cells are further injected into the abdominal cavity of mice, producing and obtaining mouse ascites fluid containing sumo tag monoclonal antibody. This monoclonal antibody occurs as an unconjugated IgG1. Its purity is greater than 95% using protein G purified. The target sumo tag is commonly used as an N-terminus fusion sequence in yeast to increase the expression and solubility of the wanted recombinant proteins. And this anti-sumo tag monoclonal antibody has been validated for use in the ELISA and WB assays.

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