Myc tag Monoclonal Antibody

Code: CSB-MA000041M0m

Rate （5.0）
Review （4）
Citation （2）

Size:

50μg100μg

US$120

Quantity:

Species Reactivity: N/A

Raised in: Mouse

Application: ELISA, WB, IF, IP

Send an Inquiry

Start an on-line Chat

Online ordering is currently available for U.S. customers only. For orders outside the U.S., please kindly submit an inquiry or start a chat with us.

Product Details
Customer Reviews and Q&A
Target Background

Product Details

Raised in

Mouse

Species Reactivity

N/A

Immunogen

EQKLISEEDL (Myc) synthetic peptide conjugate to KLH

Conjugate

Non-conjugated

Isotype

IgG1

Clone No.

5F92F9

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Concentration

It differs from different batches. Please contact us to confirm it.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB, IF, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:100-1:300
IP	1:1000-1:1500

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

WB: Mouse anti Myc-tagged fusion protein Monoclonal antibody at 1.6μg/ml
Lane 1: Recombinant Myc-tagged fusion protein at 50ng
Lane 2: Recombinant Myc-tagged fusion protein at 25ng
Lane 3: Recombinant Myc-tagged fusion protein at 12.25ng
Lane 4: Recombinant Myc-tagged fusion protein at 6.25ng
Lane 5: Recombinant Myc-tagged fusion protein at 3.125ng
Lane 6: Recombinant Myc-tagged fusion protein at 1.5625ng
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 50 kd
Observed band size: 50 kd
Immunofluorescence staining of transfected HEK293 cells with CSB-MA000041M0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunoprecipitating MYC-tag with transfected HEK293
Lane 1: Mouse control IgG (1µg) instead of CSB-MA000041M0m in transfected HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-MA000041M0m (6µg) + transfected HEK293 whole cell lysate (1mg)
Lane 3: Transfected HEK293 whole cell lysate (10µg)

Description

Myc tag monoclonal antibody CSB-MA000041M0m was produced in the mouse immunized by using the EQKLISEEDL (Myc) synthetic peptide conjugate to KLH as the immunogen. The target peptide Myc is a popular short peptide tag (EQKLISEEDL) derived from the c-myc gene product and recognized by numerous commercial antibodies as a tag. This tag can be added to the C- or N-terminus by recombinant DNA technology and can be used for affinity chromatography and for isolating protein complexes with multiple subunits.
This Myc tag monoclonal antibody was tested in the ELISA, WB, IF and IP. The non-conjugated IgG1 got purified by protein G and reached up to 95% in purity. It can be used for detection and expression of Myc tag fusion expression proteins, intracellular localization, and purification, qualitative or quantitative detection of Myc fusion-expressed proteins and the like. It doesn’t have species restricted.

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 4 reviews

Submit a Review here

Applications : Western Blot (WB)

Sample type: Cells

Sample dilution: 1:500

Review: Antibody specificity is very good.

By Anonymous

Applications : Western Blot (WB)

Sample type: HEK293 cell lysate

Sample dilution: 1:1000

Review: Antibody specificity is very good.

By Anonymous

Applications : Immunoblotting

Sample dilution: 1:5000

Review: Since YFPN and YFPC are fused to Myc and HA tags, respectively, NbRbgA:YFPN and cpRPL:YFPC proteins were detected by immunoblotting using anti-Myc and anti-HA antibodies, respectively.

By Anonymous

Applications : WB

Sample type: Mouse HeLa Kyoto cells

Sample dilution: 1:1000

Review: Since YFPN and YFPC are fused to Myc and HA tags, respectively, NbRbgA:YFPN and cpRPL:YFPC proteins were detected by immunoblotting using anti-Myc and anti-HA antibodies, respectively.

By Anonymous

Citations

The double-edged sword of endoplasmic reticulum stress in uremic sarcopenia through myogenesis perturbation Jheng JR.et al,/,2018
Borealin-nucleosome interaction secures chromosome association of the chromosomal passenger complex Abad M A, et al,/,2019

Target Background

Promotions

Email: support@cusabio.com
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

How to Choose an Antibody for Scientific Research?How to Choose? Polyclonal, Monoclonal, or Recombinant Antibody?The difference between primary antibody and secondary antibod How to Choose the Right Secondary Antibody?How to Validate An Antibody？How to Choose the Loading Control Antibodies?How to Choose An Appropriate Isotype Control Antibody?How to Choose Monoclonal Antibody Technology? A Definitive Guide The Overview of Recombinant Antibody A Comprehensive Guide to Monoclonal Antibody Production