GST Monoclonal Antibody

Code: CSB-MA000031M0m
Size:
50μg
50μg100μg
US$120
Quantity:
Species Reactivity: All
Raised in: mouse
Application: ELISA, WB
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Product Details

Raised in
mouse
Species Reactivity
All
Immunogen
Recombinant GST Protein
Conjugate
Non-conjugated
Isotype
IgG2b
Clone No.
2A81D2
Buffer
Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Concentration
It differs from different batches. Please contact us to confirm it.
Form
liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Tested Applications
ELISA, WB
Recommended Dilution
ApplicationRecommended Dilution
WB1:500-1:5000
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Datasheet & COA
Images
  • Western blot
    All lanes : Recombinant GST Protein at 30ng/ml
    Line 1: Mouse Anti-GST monoclonal antibody at 1:1000
    Line 2: Mouse Anti-GST monoclonal antibody at 1:2000
    Line 3: Mouse Anti-GST monoclonal antibody at 1:5000
    Line 4: Mouse Anti-GST monoclonal antibody at 1:10000
    Line 5: Mouse Anti-GST monoclonal antibody at 1:20000
    Line 6: Mouse Anti-GST monoclonal antibody at 1:40000
    Line 7: Mouse Anti-GST monoclonal antibody at 1:80000
    Predicted band size : 28 kd
    Observed band size : 28 kd
Description
GST monoclonal antibody CSB-MA000031M0m was produced in the mouse immunized by using the Recombinant GST Protein as the immunogen. GST can be used to purify a protein by inserting its coding sequence next to your protein of interest. They will then be expressed as a fusion protein after transcription and translation. The GST tag system has the characteristics of high protein expression yield, convenient purification of expression products, and favorable preparation of GST antibodies.
This GST Monoclonal Antibody was tested in the WB and ELISA applications. The non-conjugated IgG2b got purified by protein G and reached up to 95% in purity. It can be used for detection and expression of GST-tag fusion expression proteins, intracellular localization, and purification, qualitative or quantitative detection of His fusion-expressed proteins and the like. It doesn’t have species restricted.

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 3 reviews

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Applications : WB

Sample dilution: 1: 3000

Review: The in vitro translated emerin deletion mutants were subjected to GST pull-down assays with purified GST and GST-NICD. Bound proteins were analyzed by western blotting with anti-HA antibody. The purified proteins were subjected to western blot analysis with anti-GST antibody.

By Anonymous

Applications : GST pull-down assay

Review: The in vitro translated emerin deletion mutants were subjected to GST pull-down assays with purified GST and GST-NICD. Bound proteins were analyzed by western blotting with anti-HA antibody. The purified proteins were subjected to western blot analysis with anti-GST antibody.

By Anonymous

Applications : GST Pull-Down Assay

Review: The in vitro translated emerin deletion mutants were subjected to GST pull-down assays with purified GST and GST-NICD. Bound proteins were analyzed by western blotting with anti-HA antibody. The purified proteins were subjected to western blot analysis with anti-GST antibody.

By Anonymous

Target Background

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