GFP Monoclonal Antibody

CSB-MA000051M0m

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Review （3）
Citation （3）

Size:

50μg100μg

US$120

Quantity:

Species Reactivity: N/A

Raised in: mouse

Application: ELISA,WB,IP

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Product Details
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Target Background

Product Details

Raised in

mouse

Species Reactivity

N/A

Immunogen

Recombinant GFP Protein

Conjugate

Non-conjugated

Isotype

IgG2b

Clone No.

10B4E3

Buffer

Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Concentration

It differs from different batches. Please contact us to confirm it.

Form

liquid

Tested Applications

ELISA,WB,IP

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IP	1:200-1:2000

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Protocols

ELISA Protocol Western Blotting(WB) Protocol Immunoprecipitation (IP) Protocol

Datasheet & COA

Antibody FAQs

Images

WB: Mouse Anti-GFP monoclonal antibody at 0.1μg/ml
Line 1:HEK-293 cell lysate
Line 2:GFP transfected HEK-293 cell lysate
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size : 30kd
Observed band size : 30kd
IP:Result of anti-GFP-tag Monoclonal antibody
Line 1:Control IgG
Line 2:Precipitateing GFP-tagged fusion protein
Line 3:GFP Transfected HEK-293 cells lysate
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size : 30kd
Observed band size : 30kd

Description

GFP monoclonal antibody CSB-MA000051M0m was produced in the mouse immunized by using the Recombinant GFP Protein as the immunogen. The target protein GFP is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. GFP can be excited by the 488 nm laser line and is optimally detected at 510 nm.
This GFP Monoclonal Antibody was tested in the ELISA, WB and IP. The non-conjugated IgG2b got purified by protein G and reached up to 95% in purity. Anti-GFP antibodies provide a convenient method for visualizing GFP, especially when amplification of the fluorescent protein of interest is necessary to overcome a dim or degraded signal. It doesn’t have species restricted.