6*His Monoclonal Antibody

Code: CSB-MA000011M0m

Rate （5.0）
Review （1）
Citation （4）

Size:

50μl100μl

US$120

Quantity:

Species Reactivity: N/A

Raised in: Mouse

Application: ELISA, WB

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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Alternative Names

6*His, 6*His, His Tag, 6histag, 6*His, HHHHHH, HIS, His Tag

Target Names

6*His

Raised in

Mouse

Species Reactivity

N/A

Immunogen

HHHHHH(6x His) synthetic peptide conjugate to KLH

Conjugate

Non-conjugated

Isotype

IgG2a

Clone No.

3G5H8

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Concentration

It differs from different batches. Please contact us to confirm it.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: HIS fusion protein 1 at 20ng, 10ng, 5ng, 2.5ng
All lanes: HIS antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
Western Blot
Positive WB detected in: HIS fusion protein 2 at 20ng, 10ng, 5ng, 2.5ng
All lanes: HIS antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa

Description

The 6His Monoclonal Antibody specifically reacts to recombinant proteins containing the 6xHis-Tag. The 6*His tag is widely used in recombinant protein isolation and purification, and this antibody is a valuable tool for researchers who need to detect or isolate proteins that have been tagged with the 6*His sequence. This antibody is immunized with a 6*His synthetic peptide conjugated to KLH and produced in mouse.

The 6*His Monoclonal Antibody underwent protein A mediated purification and was purified to a high degree of purity, >95%. It is available in liquid form and belongs to the IgG2a isotype. This antibody has been validated in both ELISA and WB assays, making it a useful tool for the detection and quantification of recombinant proteins containing the 6*His-Tag. Its high specificity and purity ensure reliable and reproducible results. This antibody has been cited in a paper by Dominguez G.et al. in 2018, increasing its reliability and usefulness.

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

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Applications : WB

Sample type: Aliquots of the cultures

Review: Validation of anti-SphK2 antibody. Escherichia coli strain BL21 overexpressing His-tagged full length SphK1, His-tagged full length SphK2, and human brain lysate were analyzed by Western blotting using anti-Histidine.

By Anonymous

Citations

Neuronal sphingosine kinase 2 subcellular localization is altered in Alzheimer's disease brain Dominguez G.et al,/,2018
Lsr2 acts as a cyclic di-GMP receptor that promotes keto-mycolic acid synthesis and biofilm formation in mycobacteria W Wang,/,2024
cDNA cloning, prokaryotic expression, and functional analysis of squalene synthase (SQS) in Camellia vietnamensis Huang Jiani Dai,/,2023
GSH as an A‐A Type Allosteric Activator of PKM2: Modulating Cancer Cell Homeostasis and Ferroptosis Susceptibility TJ Chen, CJ Lo, MJ Wu, WY Sit, HY Hsu,/,2025

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