His-Tag Monoclonal Antibody

Code: CSB-MA000159

Rate （5.0）
Review （4）
Citation （4）

Size:

50μg100μg

US$100

Quantity:

Species Reactivity: N/A

Raised in: Mouse

Application: ELISA, WB, IF, IP

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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Target Names

His-Tag

Raised in

Mouse

Species Reactivity

N/A

Immunogen

Synthetic Peptide

Conjugate

Non-conjugated

Isotype

IgG

Buffer

PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Concentration

It differs from different batches. Please contact us to confirm it.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB, IF, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:50-1:200
IP	1:200-1:2000

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

IP antibody use: 5µg His Mouse IgG1 per ml Lysate, WB 1:3000
Lane 1: untransfected 293 cell lysate
Lane 2: transfected 293 cell lysate with His-tag fusion protein
Lane 3: IP (untransfected 293 + anti-His mAb + Protein G agarose)
Lane 4: IP (transfected 293 + normal Mouse IgG + Protein G agarose)
Lane 5: IP (transfected 293 + anti-His mAb + Protein G agarose)
Lane 6: Recombinant protein (E.coli)
IF analysis of 293 cells transfected with a His-tag protein,1:1000 dilution (blue DAPI, red anti-His)
2µg His fusion protein + Primary antibody dilution at 1) 1:5000 2) 1:10000

Description

His-Tag monoclonal antibody CSB-MA000159 was produced in mouse immunized by using Synthetic Peptide as the immunogen. The immunogen is a short peptide. It is specifically designed for the adsorptive purification of recombinant proteins. The relatively small size of His tags makes its integration into expression vectors extremely easy.
This His-Tag Monoclonal Antibody was tested in the WB, IF, IP and ELISA applications. An efficient detection and purification system based on fusion proteins can be established using the His tag. It can be used for detection and expression of His-tag fusion expression proteins, intracellular localization, and purification, qualitative or quantitative detection of His fusion-expressed proteins and the like. It doesn’t have species restricted.

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 4 reviews

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Applications : Pull-down assay

Review: Competitive binding assays of CgRuby1 and CgRuby2Short binding to CgbHLH1. The mixture of HIS-CgRuby1 and FLAG CgRuby2Short was added to immobilized GSTCgbHLH1. The precipitates were detected using western blot analysis with anti-HIS, anti-FLAG or anti-GST antibodies. The gradient indicates the increasing amount of FLAG-CgRuby2Short. These experiments were repeated independently twice with similar results.

By Anonymous