E-Tag Monoclonal Antibody

CSB-MA000151M0m

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Size:

50μl100μl

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Species Reactivity: All

Raised in: Mouse

Application: ELISA, WB, IP

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Product Details
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Target Background

Product Details

Alternative Names

E-Tag

Target Names

E-Tag

Raised in

Mouse

Species Reactivity

All

Immunogen

GAPVPYPDPLEPRC synthetic peptide conjugate to KLH

Conjugate

Non-conjugated

Isotype

IgG1

Clone No.

11H12B3

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Concentration

It differs from different batches. Please contact us to confirm it.

Form

Liquid

Tested Applications

ELISA, WB, IP

Recommended Dilution

Application	Recommended Dilution
ELISA	1:4000-1:8000
WB	1:1000-1:320000
IP	1μg-5μg

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Protocols

ELISA Protocol Western Blotting(WB) Protocol Immunoprecipitation (IP) Protocol

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: E-tagged fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng
All lanes: E-Tag antibody at 1:1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
Western Blot
Positive WB detected in: E-tagged fusion protein
All lanes: E-Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
Immunoprecipitating E-Tag in 293F transfected whole cell lysate
Lane 1: Mouse control IgG (1µg) instead of CSB-MA000151M0m in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-MA000151M0m (5µg) + 293F transfected whole cell lysate (500µg)
Lane 3: 293F transfected whole cell lysate (20µg)
ELISA E-Tag antibody in E-tagged fusion protein and Control protein (no E-tag)
Antigen at dilution of 1µg/ml
E-Tag antibody at (0-0.15µg)
Goat polyclonal to Mouse IgG at 1/50000 dilution
Western Blot
Positive WB detected in: 1-3 lanes: E‐tagged fusion protein, 4-5 lanes: Recombinant protein without E-tagged, 6 lane: BSA-E-Tag
All lanes: E-Tag antibody at 1:1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 30, 68, 55, 67-157kDa
Observed band size: 30, 68, 55, 67-157kDa

Description

The monoclonal anti-E-Tag (IgG1 isotype) antibody is produced from the hybridomas fused by the myeloma cells and mouse splenocytes. The splenocytes are isolated from the mouse immunized with the E-tag synthetic peptide conjugated to KLH. This unconjugated E-Tag monoclonal antibody is purified through protein G with a purity of more than 95%. It can react with all E-Tag-fused proteins. And it is amenable to ELISA, WB, and IP applications.

E-Tag is a short amino acid sequence (GAPVPYPDPLEPR) that is frequently used as an epitope tag in molecular biology experiments to facilitate the detection and purification of recombinant proteins. The E-Tag sequence is genetically fused to the protein of interest, and antibodies or affinity resins that specifically recognize the E-Tag can be used to detect or purify the tagged protein. This allows researchers to study the expression, localization, and function of the protein in cells or tissues.