ACTB Monoclonal Antibody

Code: CSB-MA000091M2m

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Size:

50μl100μl

US$120

Quantity:

Species Reactivity: Human, Mouse, Rat, Rabbit

Raised in: Mouse

Application: ELISA, WB

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Product Details
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Target Background

Product Details

Target Names

ACTB

Raised in

Mouse

Species Reactivity

Human, Mouse, Rat, Rabbit

Immunogen

A synthesized peptide derived from human Beta-Actin (1-50AA)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

IgG1

Clone No.

6D8D8

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Concentration

It differs from different batches. Please contact us to confirm it.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:10000-1:1280000

Protocols

ELISA Protocol Western Blotting(WB) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Mouse heart tissue, Mouse kidney tissue, Rat heart tissue, Rat skeletal muscle tissue, Rabbit lung tissue, Rabbit liver tissue
All lanes: ACTB antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
Exposure time：5min
Western Blot
Positive WB detected in: 293T whole cell lysate, Zebrafish tissue
All lanes: ACTB antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
Exposure time：5min
Western Blot
Positive WB detected in: Mouse heart tissue, Mouse liver tissue, Mouse kidney tissue, Mouse spleen tissue, Mouse skeletal muscle tissue
All lanes: ACTB antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
Exposure time：5min
Western Blot
Positive WB detected in: Rat heart tissue, Rat liver tissue, Rat kidney tissue, U87 whole cell lysate Rat skeletal muscle tissue
All lanes: ACTB antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
Exposure time：5min
Western Blot
Positive WB detected in: Rabbit heart tissue, Rabbit liver tissue, Rabbit lung tissue, Rabbit small intestine tissue, Rabbit skeletal muscle tissue, A549 whole cell lysate, MCF-7 whole cell lysate
All lanes: ACTB antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
Exposure time：5min
Western Blot
Positive WB detected in: Hela whole cell lysate at 40μg, 20μg, 10μg, 5μg, 2.5μg, 1.25μg, 0.625μg, 0.3125μg
All lanes: ACTB antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
Exposure time：5min
Western Blot
Positive WB detected in: 20μg Hela whole cell lysate
ACTB antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000, 1:1280000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
Exposure time：5min

Description

The ACTB monoclonal antibody is created using the synthesized peptide derived from human Beta-Actin (1-50aa) to immunize the mouse, with hybridoma cells being generated by fusing mouse B cells (isolated from the immunized mouse) with myeloma cells. The hybridoma cells that produce the ACTB antibody are then selected and cultured, and the ACTB monoclonal antibody is purified from the mouse ascites using protein G affinity chromatography. This unconjugated IgG1 antibody is highly specific to the ACTB proteins from four species including human, mouse, rat, and rabbit. It can be used in ELISA and WB assays.

ACTB, also known as beta-actin, is a protein that plays a structural role in the cytoskeleton of eukaryotic cells. It is involved in maintaining cell shape, adhesion, and motility, and is also important for intracellular transport and cytokinesis. Additionally, ACTB is involved in gene transcription and chromatin remodeling and has been shown to interact with a variety of proteins involved in signaling pathways and cellular processes.

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