NF-κB (Luc) HEK293 Reporter Cell

CSB-SC6123HU

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Size:

1 vial contains approximately 5x10⁶ cells in 1 ml

US$5000

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Target Background

Product Details

Growth Properties

Adherent

Selection Marker

hygromycin

Culture Medium

DMEM+10% FBS+100μg/ml hygromycin

Tag Info

Tag-Free

Buffer

90% FBS + 10% DMSO

Storage

Frozen in liquid nitrogen or -80°C

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Protocols

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The NF-kB (Luc) HEK293 Reporter Cell was engineered with the NF-kB response element driving luciferase expressing systems. The receptors expressing endogenously or transfected on this reporter cell were activated by corresponding ligands binding, transducing intracellular signals resulting in NF-kB-RE mediated luminescence.
Response to human TNFα protein (RLU). The NF-kB (Luc) HEK293T Reporter Cell was stimulated with serial dilutions of human TNFα protein. The EC50 was approximately 0.9507 ng/mL.
Response to human TNFα protein (Fold). The NF-kB (Luc) HEK293T Reporter Cell was stimulated with serial dilutions of human TNFα protein. The max induction fold was approximately 611.
Passage stability analysis by Signaling Bioassay. The continuously growing NF-kB (Luc) HEK293T Reporter Cell was stimulated with serial dilutions of human TNFα protein. Human TNFα protein stimulated response demonstrates passage stabilization (fold induction and EC50) across passage 9-30.