NF-κB (Luc) HEK293 Reporter Cell

Code: CSB-SC6123HU

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Size:

1 vial contains approximately 5x10⁶ cells in 1 ml

US$5000

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Product Details

Growth Properties

Adherent

Selection Marker

hygromycin

Culture Medium

DMEM+10% FBS+100μg/ml hygromycin

Tag Info

Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Buffer

90% FBS + 10% DMSO

Storage

Frozen in liquid nitrogen or -80°C

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Images

The NF-kB (Luc) HEK293 Reporter Cell was engineered with the NF-kB response element driving luciferase expressing systems. The receptors expressing endogenously or transfected on this reporter cell were activated by corresponding ligands binding, transducing intracellular signals resulting in NF-kB-RE mediated luminescence.
Response to human TNFα protein (RLU). The NF-kB (Luc) HEK293T Reporter Cell was stimulated with serial dilutions of human TNFα protein. The EC50 was approximately 0.9507 ng/mL.
Response to human TNFα protein (Fold). The NF-kB (Luc) HEK293T Reporter Cell was stimulated with serial dilutions of human TNFα protein. The max induction fold was approximately 611.
Passage stability analysis by Signaling Bioassay. The continuously growing NF-kB (Luc) HEK293T Reporter Cell was stimulated with serial dilutions of human TNFα protein. Human TNFα protein stimulated response demonstrates passage stabilization (fold induction and EC50) across passage 9-30.

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