DPEP3 Recombinant Monoclonal Antibody

Dipeptidase 3 (DPEP3) belongs to the membrane-bound dipeptidase family, enzymes that play important roles in peptide metabolism and cellular homeostasis. As a relatively understudied member of this enzyme class, DPEP3 represents an emerging target for researchers investigating dipeptidase biology and its implications in cell biology.

This recombinant monoclonal antibody, clone 5H3, offers the reproducibility and consistency that demanding research applications require. Because the antibody sequence is defined and produced through recombinant expression in mouse host cells, you can expect reliable performance across experiments and between lots, eliminating the variability often encountered with traditional hybridoma-derived antibodies. The mIgG1 isotype and affinity-chromatography purification ensure high purity suitable for sensitive detection methods.

Functional ELISA validation demonstrates the antibody's robust binding characteristics, with EC50 values in the low nanomolar range for both human DPEP3 (6.841–7.498 ng/mL) and Macaca fascicularis DPEP3 (7.817–8.936 ng/mL). This cross-species reactivity provides flexibility for researchers working with primate models or conducting comparative studies. The recommended ELISA dilution range of 1:5000 to 1:50000 reflects the antibody's high affinity, allowing economical use across multiple experiments.

Supplied in a stabilizing buffer containing 50% glycerol and PBS at pH 7.4, this unconjugated antibody maintains activity during long-term storage at -20°C or -80°C. The liquid format enables straightforward integration into your existing protocols without reconstitution steps. For investigators exploring dipeptidase function in cell biology contexts, this antibody provides a sequence-defined, validated tool for reliable DPEP3 detection.