CCR5 Recombinant Monoclonal Antibody

CCR5, or C-C chemokine receptor type 5, serves as a critical G protein-coupled receptor that mediates immune cell trafficking and inflammatory responses. Beyond its physiological role in chemokine signaling, CCR5 has gained particular prominence as the primary coreceptor for HIV-1 entry into host cells, making it an intensively studied target in infectious disease research. The receptor's involvement extends across multiple disease contexts, including cancer progression, cardiovascular pathology, and neuroinflammatory conditions, where its expression patterns and signaling dynamics offer valuable mechanistic insights.

This recombinant monoclonal antibody, clone 11D4, provides researchers with a sequence-defined reagent that delivers the lot-to-lot consistency essential for longitudinal studies and reproducible experimental outcomes. Produced using recombinant technology and purified by affinity chromatography, this human IgG1 isotype antibody was raised against recombinant human CCR5 protein, ensuring precise epitope targeting for human samples.

Functional validation demonstrates robust binding activity, with ELISA testing showing an EC50 of 1.099-1.287 ng/mL when binding to immobilized human CCR5, while virus-like particle controls confirmed specificity. For flow cytometry applications, dilutions of 1:50-1:200 are recommended, enabling flexible optimization for cell surface detection studies examining CCR5 expression on immune cell populations.

The antibody is supplied in a liquid format containing PBS with 50% glycerol and 0.03% Proclin 300 preservative, ready for immediate use in your experimental workflows. Whether investigating HIV-1 coreceptor biology, characterizing immune cell phenotypes, or exploring CCR5's role in tumor microenvironments and inflammatory signaling cascades, this reagent offers the reliability and performance that demanding research applications require.