MADCAM1 Antibody

Code: CSB-PA618776HA01HU

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US$83

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Species Reactivity: Human

Raised in: Rabbit

Application: ELISA, WB, IF

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Product Details
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Target Background

Product Details

Uniprot NO.

Q13477

Target Names

MADCAM1

Alternative Names

Addressin mucosal antibody; hMAdCAM 1 antibody; hMAdCAM-1 antibody; MACAM1 antibody; MADCA_HUMAN antibody; MAdCAM 1 antibody; MAdCAM-1 antibody; Madcam1 antibody; Mucosal addressin cell adhesion molecule 1 antibody; Mucosal vascular addressin cell adhesion molecule 1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Mucosal addressin cell adhesion molecule 1 protein (19-317AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:50-1:200

Protocols

ELISA Protocol Western Blotting(WB) Protocol Immunofluorescence (IF) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Immunofluorescent analysis of HepG2 cells using CSB-PA618776HA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Western Blot
Positive WB detected in: HepG2 whole cell lysate
All lanes: MADCAM1 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 41, 29, 32, 22 kDa
Observed band size: 41 kDa

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function(From Uniprot)

Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.

Gene References into Functions

Both the ACT-1 assay and the MAdCAM-1 assay demonstrated acceptable reproducibility and repeatability. The assays were sufficiently stable to allow for clinical use. During clinical testing the assays demonstrated that vedolizumab was able to saturate peripheral cells at all doses tested. PMID:25908521
Doxo-induced apoptosis has a role in inhibiting hepatocellular carcinoma by targeting Madcam1 and AKT and blocking protein translation initiation PMID:26124182
MADCAM1 is up-regulated in liver cirrhosis with expression on peribiliary vascular plexus and lymphoid aggregates PMID:23839340
Domain 1 of mucosal addressin cell adhesion molecule has an I1-set fold and a flexible integrin-binding loop. PMID:23297416
Findings suggest that the inactive alpha4beta7 and alpha4beta7 activated by different stimuli have distinct conformations with different structural requirements for MAdCAM-1 binding. PMID:21296888
Ca2+ is essential for cell tethering in flow and rolling interactions through alpha 4 integrins with MAdCAM-1 as substrate; Mg2+ promotes firm adhesion of cells on MAdCAM-1 but with much lower tethering efficiency in shear flow. PMID:11705388
A crystal structure for the two extracellular amino-terminal domains of human MAdCAM-1 PMID:11807247
more extensive expression of MAdCAM-1 in Crohn's disease (CrD), than in ulcerative colitis, which could contribute not only to mucosal inflammation, but also to transmural inflammation in CrD. PMID:12100519
MAdCAM-1/integrin alpha4beta7 homing system may participate in gastric inflammation in response to H pylori-infection and contributes to MALT formation, typically leading to the development of nodular gastritis. PMID:14669317
a marker of graft rejection in small intestine transplantation PMID:15050155
PI3-K/Akt is involved in the gut-specific differentiation of human intestinal microvascular endothelial cells, which results in expression of the mucosal addressin MAdCAM-1 PMID:15483224
Immobilized CCL25 and CCL28 are both able to trigger integrin alpha(4)beta(7)-dependent lymphocyte arrest on MAdCAM-1 under shear flow. PMID:18308860
mucosal addressin cell adhesion molecule 1 may represent an important determinant for the generation of mucosal damage in celiac disease PMID:19157500
MADCAM1 gene polymorphisms may be associated with the risk of chronic GVHD and may, also, affect mortality related to aHSCT. PMID:19286444

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Subcellular Location

Membrane; Single-pass type I membrane protein.

Tissue Specificity

Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ov

Database Links

HGNC: 6765

UNIGENE: Hs.102598

KEGG: hsa:8174

STRING: 9606.ENSP00000215637

OMIM: 102670

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