GRK3 Antibody

CSB-PA001395LA01HU

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US$83

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Species Reactivity: Human

Raised in: Rabbit

Application: ELISA, WB, IHC, IF

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Product Details
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Target Background

Product Details

Uniprot NO.

P35626

Target Names

GRK3

Alternative Names

ADRBK2 antibody; Adrenergic; beta; receptor kinase 2 antibody; ARBK2_HUMAN antibody; BARK2 antibody; Beta adrenergic receptor kinase 2 antibody; Beta ARK 2 antibody; Beta-adrenergic receptor kinase 2 antibody; Beta-ARK-2 antibody; EC 2.7.11.15 antibody; G protein coupled receptor kinase 3 antibody; G-protein-coupled receptor kinase 3 antibody; GRK3 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Beta-adrenergic receptor kinase 2 protein (343-480AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Protocols

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: Hela whole cell lysate
All lanes: GRK3 antibody at 2μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 80 kDa
Observed band size: 80 kDa
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA001395LA01HU at dilution of 1:100
Immunofluorescence staining of HepG2 cells with CSB-PA001395LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Description

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Target Background

Function

Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors.

Gene References into Functions

Study found that GRK3 was significantly overexpressed in 162 pairs of colon cancer tissues than in the matched noncancerous mucosa. These data show that aberrant expression of GRK3 plays an important role in promoting colon cancer progression through enhanced proliferation and reduced apoptosis. PMID:29445249
hat expression of GRK3 was down-regulated in pancreatic ductal adenocarcinoma and was an independent prognostic factor PMID:29254792
GRK3 is a new critical activator of neuroendocrine phenotypes and mediator of CREB activation in promoting neuroendocrine differentiation of prostate cancer cells. PMID:27191986
effects of GRK3 modulation appear to be specific to chemokine-mediated migration behaviors without influencing tumor cell proliferation or survival PMID:27049755
data are consistent with the possibility that oncogenes can induce cellular stiffness via an HDAC6-induced reorganization of the vimentin intermediate filament network PMID:24434559
In oral squamous cell carcinomas, malignant cells and surrounding tissue overexpress the ADRBK2 gene. PMID:21916780
A reduced cortical concentration of GRK3 in schizophrenia (resembling that in aging) may result in altered G protein-dependent signaling, thus contributing to prefrontal deficits in schizophrenia. PMID:21784156
GRK3 is a negative regulator of cell growth whose expression is preferentially reduced in glioblastoma of the classical subtype as a consequence of activity in primary gliomagenic pathways. PMID:22086906
mRNA levels for GRK3 were inversely correlated with systolic and diastolic blood pressure (day, night and 24 h), which suggests a protective role for GRK3 in the regulation of human blood pressure PMID:20216086
we found no evidence of altered levels of acetylated histone H3 at the affected allele compared to the common allele PMID:19766236
dysregulation in GRK3 expression alters signaling desensitization, and thereby predisposes to the development of bipolar disorder PMID:12808434
the CRH-R1alpha carboxyl tail is important for regulation of receptor activity by G protein-coupled receptor kinase PMID:15498832
ATP stimulation leads to GRK3 phosphorylation of P2X(7) receptor. PMID:15728711
No support for a major role for GRK3 gene promoter variants in cocaine addiction. PMID:17621168
Dysregulation in GRK3 expression alters signaling desensitization and thereby predisposes to the development of bipolar disease. PMID:18075471
role for GRK3 in regulating CXCR4 attenuation and have provided a mechanistic link between the GRK3 pathway and the CXCR4-related WHIM(WT) disorder. PMID:18274673
The G-384A variant may alter binding of Sp1/Sp4 transcription factors resulting in an increase in gene transcription and an increase in vulnerability to bipolar disorder. PMID:18359007