GPR101 Antibody

CSB-PA846682LA01HU

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20μg50μg100μg

US$83

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Species Reactivity: Human

Raised in: Rabbit

Application: ELISA, WB, IF

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Product Details
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Target Background

Product Details

Uniprot NO.

Q96P66

Target Names

GPR101

Alternative Names

GPR101; Probable G-protein coupled receptor 101

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Probable G-protein coupled receptor 101 protein (218-399AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IF	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Protocols

ELISA Protocol Western Blotting(WB) Protocol Immunofluorescence (IF) Protocol

Datasheet & COA

Antibody FAQs

Images

Western blot
All lanes: GPR101 antibody at 6µg/ml
Lane 1: MCF-7 whole cell lysate
Lane 2: U87 whole cell lysate
Lane 3: Caco-2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa
Immunofluorescent analysis of U251 cells using CSB-PA846682LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Description

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Target Background

Function

Orphan receptor.

Gene References into Functions

Germline or somatic microduplications of the Xq26.3 chromosomal region, invariably involving the GPR101 gene, constitute the genetic defect leading to X-Linked Acrogigantism. GPR101 encodes a class A G protein-coupled receptor that activates the 3',5'-cyclic adenosine monophosphate signaling pathway. PMID:29678281
This study shows that different GPR101 transcripts exist and that the brain is the major site of GPR101 expression across different species, although divergent species- and temporal-specific expression patterns are evident. These findings suggest an important role for GPR101 in brain and pituitary development and likely reflect the very different growth, development and maturation patterns among species. PMID:27282544
Study showed that X-linked acrogigantism (XLAG) can result from germline or somatic duplication of GPR101. Duplication of GPR101 alone is sufficient for the development of XLAG, implicating it as the causative gene within the Xq26.3 region. The pathological features of XLAG-associated pituitary adenomas are typical and, together with the clinical phenotype, should prompt genetic testing. PMID:27245663
p.E308D variant not found in acromegaly cases PMID:26815903
This study did not identify GPR101 abnormalities as a frequent cause of growth hormone deficiency. PMID:26797872
Germline GPR101 mutations are very rare in patients with sporadic pituitary adenomas of various histotypes. PMID:26792934
X-linked acrogigantism is caused by an Xq26.3 genomic duplication and is characterized by early-onset gigantism. Also found recurrent mutation in GPR101 in some adults with acromegaly. PMID:25470569
GPR101 is a critical requirement for GnRH-(1-5) transactivation of EGFR in Ishikawa cells. PMID:24264576