Butyrly-HIST1H2BC (K5) Antibody

CSB-PA010403OA05butHU

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20μl50μl100μl

US$88

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Species Reactivity: Human

Raised in: Rabbit

Application: ELISA, WB, IF, IP, ChIP

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Product Details
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Target Background

Product Details

Uniprot NO.

P62807

Target Names

HIST1H2BC

Alternative Names

H2BC4 antibody; H2BFL antibody; HIST1H2BC; antibody; H2BC6 antibody; H2BFH antibody; HIST1H2BE; antibody; H2BC7 antibody; H2BFG antibody; HIST1H2BF; antibody; H2BC8 antibody; H2BFA antibody; HIST1H2BG; antibody; H2BC10 antibody; H2BFK antibody; HIST1H2BIHistone H2B type 1-C/E/F/G/I antibody; Histone H2B.1 A antibody; Histone H2B.a antibody; H2B/a antibody; Histone H2B.g antibody; H2B/g antibody; Histone H2B.h antibody; H2B/h antibody; Histone H2B.k antibody; H2B/k antibody; Histone H2B.l antibody; H2B/l antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Peptide sequence around site of Butyrly-Lys (5) derived from Human Histone H2B type 1-C/E/F/G/I

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Tested Applications

ELISA, WB, IF, IP, ChIP

Recommended Dilution

Application	Recommended Dilution
WB	1:100-1:1000
IF	1:10-1:100
IP	1:200-1:2000

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Protocols

ELISA Protocol Western Blotting(WB) Protocol Immunofluorescence (IF) Protocol Immunoprecipitation (IP) Protocol Native Chromatin Immunoprecipitation(ChIP) Protocol

Datasheet & COA

Antibody FAQs

Images

Western Blot
Detected samples: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h
All lanes: HIST1H2BC antibody at 1:100
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CSB-PA010403OA05butHU at 1:15, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating HIST1H2BC in HEK293 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA010403OA05butHU in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA010403OA05butHU (5µg) + HEK293 whole cell lysate (500µg)
Lane 3: HEK293 whole cell lysate (20µg)
Chromatin Immunoprecipitation Hela (10⁶, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H2BC (CSB-PA010403OA05butHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

Description

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Target Background

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.; Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.

Subcellular Location

Nucleus. Chromosome.

Protein Families

Histone H2B family

Database Links

HGNC: 4757

UNIGENE: Hs.182137

KEGG: hsa:3017

STRING: 9606.ENSP00000366962

OMIM: 602798

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