| Application | Recommended Dilution |
|---|---|
| WB | 1:200-1:1000 |
| IHC | 1:20-1:200 |
Western blot
All lanes: ABCF2 antibody at 4μg/ml
Lane 1: 293T whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: NIH/3T3 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 72, 73 kDa
Observed band size: 72 kDa
Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA871400ESR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA871400ESR2HU at dilution of 1:100
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