Abbreviation
Recombinant Human SPP1 protein (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA.Immobilized Human SPP1 at 2 μg/mL can bind Anti-SPP1 recombinant antibody(CSB-RA022603MA1HU).The EC50 is 4.929-5.425 ng/mL.
Alternative Names
Osteopontin; Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin
Species
Homo sapiens (Human)
Expression Region
17-314aa
Target Protein Sequence
IPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQKQNLLAPQNAVSSEETNDFKQETLPSKSNESHDHMDDMDDEDDDDHVDSQDSIDSNDSDDVDDTDDSHQSDESHHSDESDELVTDFPTDLPATEVFTPVVPTVDTYDGRGDSVVYGLRSKSKKFRRPDIQYPDATDEDITSHMESEELNGAYKAIPVAQDLNAPSDWDSRGKDSYETSQLDDQSAETHSHKQSRLYKRKANDESNEHSDVIDSQELSKVSREFHSHEFHSHEDMLVVDPKSKEEDKHLKFRISHELDSASSEVN
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
Storage
Store at -20°C/-81°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA.Immobilized Human SPP1 at 2 μg/ml can bind Anti-SPP1 recombinant antibody(CSB-RA022603MA1HU).The EC50 is 4.929-5.425 ng/mL.
Description
Osteopontin mediates cell adhesion, migration, and survival across tumor microenvironments and inflammatory contexts, making quantitative assessment of its binding interactions essential for mechanistic studies. This full-length mature protein (aa 17–314) demonstrates specific antibody recognition with an EC50 of 4.929–5.425 ng/mL in functional ELISA, providing a validated reagent for antibody development workflows including epitope mapping, hybridoma screening, and immunoassay standardization. Mammalian expression preserves the native post-translational modifications critical to osteopontin's integrin-binding and CD44-binding functions, supporting cell-based adhesion assays, migration studies with cancer cell lines, and signaling pathway investigations of FAK, PI3K/AKT, and NF-κB activation in tumor biology models. The combination of >95% purity and <1.0 EU/μg endotoxin satisfies the quality thresholds commonly required for immune cell co-culture experiments where LPS contamination would confound osteopontin-driven macrophage polarization or T-cell activation readouts.
